pbpI [2018-02-16 18:32:10]

[SW|sporulation]-specific penicillin-binding protein 4b

locus: BSU27310

pI: 9.01

mw: 65.35 kDa

protein length: 584 aa

gene length: 1752 bp

function: unknown

product: penicillin-binding protein 4b

essential: no

synonyms: yrrR,pbpI

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,789,686 → 2,791,440

The protein

Protein family

• transpeptidase family (according to Swiss-Prot)

Structure

• [PDB|3OC2] (PBP3 from Pseudomonas aeruginosa, 23% identity) [pubmed|20974151]

[SW|Localization]

• during vegetative growth: evenly distributed along the membrane [pubmed|14731276]

Expression and Regulation

Operons

• genes: [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]-[gene|9892E5BFE90EC934A4047795BC4B328656128C09|yrrS]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|14679248,15758244], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190,16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in both cell compartments ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE] [Pubmed|14679248,15758244], [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] [Pubmed|15699190,16497325])

Biological materials

Mutant

• BKE27310 (Δ[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA
• BKK27310 (Δ[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA

GFP fusion

• 2081 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::pSG1489 (cat P_xyl-gfpa-[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]1-430) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

• 14679248,15699190,16497325,15758244,14731276,20974151