carA [Mon Aug 31 2015 13:53:36 GMT+0200 (CEST)]

carbamoyl-phosphate transferase-arginine (subunit A)

locus: BSU11230

pI: 6.21

mw: 38.00 kDa

function: biosynthesis of arginine

product: carbamoyl-phosphate transferase-arginine (subunit A)

essential: no

ec: 6.3.5.5

synonyms: cpa

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]

syns: cpa

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|AhrC regulon], [SW|CodY regulon]

The protein

Catalyzed reaction/ biological activity

• 2 ATP + L-glutamine + HCO₃^- + H₂O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)

Protein family

• glutamine amidotransferase type-1 domain (according to Swiss-Prot)

Paralogous protein(s)

• [SW|PyrAA]

Structure

• [PDB|1JDB] (from ''Escherichia coli'', 47% identity, 64% similarity) [Pubmed|10089390]

[SW|Interactions]

• [protein|search|CarA]-[protein|search|CarB]

Expression and Regulation

Operon

• ''[protein|search|argC]-[protein|search|argJ]-[protein|search|argB]-[protein|search|argD]-[protein|search|carA]-[protein|search|carB]-[protein|search|argF]'' [Pubmed|7511775]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|7511775]

Regulation

• repressed by casamino acids ([protein|search|CodY]) [Pubmed|12107147]
• repressed by arginine ([protein|search|AhrC]) [Pubmed|1312212]

Regulatory mechanism

• [protein|search|AhrC]: transcription repression [Pubmed|1312212]
• [protein|search|CodY]: transcription repression [Pubmed|24843172]

References

• 6096675,12107147,21821766,24843172