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comGA [Mon May 16 2016 17:40:10 GMT+0200 (CEST)]
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comGA [Mon May 16 2016 17:40:10 GMT+0200 (CEST)]

late competence protein, traffic ATPase, binds and transports transforming DNA, required for growth arrest of competent cells
locus
BSU24730
pI
8.00
mw
40.00 kDa
function
genetic competence
product
traffic ATPase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|genetic competence], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|ComK regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,559,007 -> 2,560,077

    Phenotype of a mutant

  • loss of [SW|genetic competence]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and transports transforming DNA
  • sequesters [SW|MreB] during expression of [Pubmed|genetic competence] to inhibit cell elongation during escape from competence [Pubmed|26091431]

    • Protein family

  • GSP E family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Arg-28, Arg-71- and Arg-330 [Pubmed|22517742]

    • [SW|Localization]

  • peripheral membrane protein [Pubmed|9723928]
  • localizes to cell poles in competent cells [Pubmed|16009133]
  • localizes to one cell pole [Pubmed|21278288]

    • [SW|Interactions]

  • [SW|ComGA]-[SW|ComGB] [Pubmed|15083159]
  • [SW|ComGA]-[SW|ComFA] [Pubmed|17630974]
  • [SW|ComEC]-[SW|ComGA] [Pubmed|17630974]
  • [protein|search|Maf]-[SW|ComGA] [Pubmed|21564336]
  • [protein|search|RelA]-[SW|ComGA], required for the inhibition of rRNA synthesis in the K-state [Pubmed|25899641]
  • [SW|MreB]-[SW|ComGA] [Pubmed|26091431]

    • Additional information

  • ComGA stability depends on [protein|search|Maf] [Pubmed|21564336]

    • Expression and Regulation

    Operon

  • ''[SW|comGA]-[SW|comGB]-[SW|comGC]-[SW|comGD]-[SW|comGE]-[SW|comGF]-[SW|comGG]-[protein|search|spoIIIL]'' [Pubmed|2507524]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2507524]

    • Regulation

  • repressed by casamino acids [Pubmed|12107147]

    • Regulatory mechanism

  • [protein|search|ComK]: transcription activation [Pubmed|8196543]

    • Biological materials

    GFP fusion

  • GP1640 ''trpC2'' ''[protein|search|ymdB]''::''spc'' P''comG-gfp'' (''cat''), available in [SW| Jörg Stülke]'s lab
  • GP1643 P''comG-gfp'' (''cat'') in the NCIB3610 background, available in [SW| Jörg Stülke]'s lab

    • References

    Reviews

  • 15083159

    • Original publications

  • 11814663,9422590,16009133,11298275,17630974,9723928,21278288,12107147,8196543,2507524,21564336,21707789,22517742,25899641,26091431