yrrL

potential terminase for peptidoglycan polymerization

locus: BSU_27370

pI: 9.65

mw: 40.34 kDa

protein length: 360 aa

gene length: 1083 bp

function: determination of peptidoglycan chain length

product: potential terminase for peptidoglycan polymerization

essential: no

synonyms: mltG

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,795,887 2,796,969

The protein

Catalyzed reaction/ biological activity

• endolytic transglycosylase [Pubmed|26507882]

Protein family

• transglycosylase MltG family (single member, according to UniProt)

Structure

• [PDB|4IIW] (from ''L. monocytogenes'', 51% identity)

Expression and Regulation

Operons

• genes: [gene|41BCD18A1C2D0C69D4445BE14D4617CC61FB23BF|yrrL]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

Biological materials

Mutant

• MGNA-A854 (yrrL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/854 NBRP B. subtilis, Japan]
• BKE27370 ([gene|41BCD18A1C2D0C69D4445BE14D4617CC61FB23BF|yrrL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGGTCTCCTCCCATCA, downstream forward: _UP4_TAAAAGGAGAGCAAAAAGCT
• BKK27370 ([gene|41BCD18A1C2D0C69D4445BE14D4617CC61FB23BF|yrrL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAGGTCTCCTCCCATCA, downstream forward: _UP4_TAAAAGGAGAGCAAAAAGCT

References

• 14651647,26507882