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lcfB [2017-8-11 16:0:3]
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lcfB [2017-8-11 16:0:3]

long-chain fatty-acid-CoA ligase, involved in surfactin production
Locus
BSU10270
Isoelectric point
5.30
Molecular weight
56.41 kDa
Protein length
513 aa Sequence Blast
Gene length
1539 bp Sequence Blast
Function
fatty acid degradation
Product
long-chain fatty-acid-CoA ligase
Essential
no
E.C.
6.2.1.3
Synonyms
yhfL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

    • Gene

    Coordinates
    1,100,980 → 1,102,521

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

    • Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • LcfA, YhfT, YngI

    • Expression and Regulation

    Operons

    Genes
    lcfB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, in CcpA regulon

    • Regulation

  • induced in the presence of long-chain fatty acids (FadR) PubMed
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    Biological materials

    Mutant

  • MGNA-B277 (yhfL::erm), available at the NBRP B. subtilis, Japan
  • available in Mohamed Marahiel's lab PubMed

    • References

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