lcfB [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

long-chain fatty-acid-CoA ligase, involved in surfactin production

locus: BSU10270

pI: 5.30

mw: 56.00 kDa

function: fatty acid degradation

product: long-chain fatty-acid-CoA ligase

essential: no

ec: 6.2.1.3

synonyms: yhfL

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of lipids]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|FadR regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,100,980 -> 1,102,521

The protein

Catalyzed reaction/ biological activity

• ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|LcfA], [protein|search|YhfT], [protein|search|YngI]

Expression and Regulation

Operon

• ''lcfB'' [Pubmed|17189250]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|17189250]

Regulation

• induced in the presence of long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
• subject to carbon catabolite repression ([protein|search|CcpA]-[PtsH|search|HPr(Ser-P)] [Pubmed|21398533]

Regulatory mechanism

• [protein|search|FadR]: transcription repression [Pubmed|17189250]
• [protein|search|CcpA]-[PtsH|search|HPr(Ser-P)]: transcription repression [Pubmed|21398533]

Biological materials

Mutant

• available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

References

• 17189250,17919287,20797616