lcfB

long-chain fatty-acid-CoA ligase, involved in surfactin production

Locus: BSU_10270

Isoelectric point: 5.30

Molecular weight: 56.41 kDa

Protein length: 513 aa

Gene length: 1542 bp

Function: fatty acid degradation

Product: long-chain fatty-acid-CoA ligase

Essential: no

E.C.: 6.2.1.3

Synonyms: yhfL

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

Gene

Coordinates: 1,100,980 1,102,521

The protein

Catalyzed reaction/ biological activity

• long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
• activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Paralogous protein(s)

• YhfT, YngI, LcfA

Structure

• 3R44 (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 35% identity) PubMed

Expression and Regulation

Operons

• Genes: lcfB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • FadR: repression, PubMed, in FadR regulon
  • CcpA: repression, in CcpA regulon

  Regulation

  • induced in the presence of long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

• MGNA-B277 (yhfL::erm), available at the NBRP B. subtilis, Japan
• available in Mohamed Marahiel's lab PubMed
• BKE10270 (lcfB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT, downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG
• BKK10270 (lcfB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT, downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG

References

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