product
DNA topoisomerase I
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation][category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins][category|SW 6|Groups of genes] → [category|SW 6.13|Quasi-essential genes][SW|Categories] containing this gene/protein
[SW|DNA condensation/ segregation], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|Efp-dependent proteins]Gene
Coordinates on the chromosome (coding sequence)
1,683,661 -> 1,685,736
Phenotypes of a mutant
essential [Pubmed|25092907]The protein
Catalyzed reaction/ biological activity
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)Protein family
prokaryotic type I/III topoisomerase family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|TopB][SW|Domains]
N-terminal [SW|TOPRIM domain] (aa 3-114) [Pubmed|9722641]Structure
[PDB|2GAI] (from ''Thermotoga maritima '', 42% identity) [Pubmed|16600296][SW|Interactions]
TopA interacts with the [SW|RNA polymerase] [Pubmed|21710567]Expression and Regulation
Operon
''topA'' [Pubmed|22383849]Additional information
[SW|translation] is likely to require [protein|search|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]Biological materials
Expression vector
pGP2060: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s labReferences
17114254,21710567,25092907,16600296,27378778,9722641 
