topA [Mon Oct 17 2016 15:01:49 GMT+0200 (CEST)]

DNA topoisomerase I

Locus: BSU16120

Isoelectric point: 9.00

Molecular weight: 78.00 kDa

Function: DNA replication

Product: DNA topoisomerase I

Essential: yes

E.C.: 5.99.1.2

Synonyms: topI

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA condensation/ segregation
• Groups of genes → Efp-dependent proteins
• Groups of genes → Quasi-essential genes

Categories containing this gene/protein

• DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

• Efp-dependent proteins

Gene

Coordinates on the chromosome (coding sequence): 1,683,661 -> 1,685,736

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)

Protein family

• prokaryotic type I/III topoisomerase family (according to Swiss-Prot)

Paralogous protein(s)

• TopB

Domains

• N-terminal TOPRIM domain (aa 3-114) PubMed

Structure

• 2GAI (from Thermotoga maritima , 42% identity) PubMed

Interactions

• TopA interacts with the RNA polymerase PubMed

Expression and Regulation

Operon

• topA PubMed

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Expression vector

• pGP2060: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

References

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