fabHA [2017-8-11 16:0:3]

beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells

Locus: BSU11330

Isoelectric point: 5.05

Molecular weight: 33.58 kDa

Protein length: 312 aa

Gene length: 936 bp

Function: fatty acid biosynthesis

Product: beta-ketoacyl-acyl carrier protein synthase III

Essential: no

E.C.: 2.3.1.180

Synonyms: yjaX

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids

Gene

Coordinates: 1,208,222 → 1,209,160

Phenotypes of a mutant

• significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species PubMed

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO₂ (according to Swiss-Prot)

Protein family

• fabH family (according to Swiss-Prot)

Paralogous protein(s)

• FabHB, one of the two proteins has to be present for viability PubMed

Structure

• 1EBL (FabH from E. coli) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis PubMed

Expression and Regulation

Operons

• Genes: fabHA-fabF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigW: sigma factor, PubMed, in SigW regulon

  Regulatory mechanism

  • FapR: repression, PubMed, in FapR regulon

  Regulation

  • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • expression of the operon is strongly reduced during biofilm formation PubMed

Biological materials

Mutant

• MGNA-B145 (yjaX::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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