fabHA [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells

Locus: BSU11330

Isoelectric point: 5.05

Molecular weight: 33.00 kDa

Function: fatty acid biosynthesis

Product: beta-ketoacyl-acyl carrier protein synthase III

Essential: no

E.C.: 2.3.1.180

Synonyms: yjaX

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids

Categories containing this gene/protein

• biosynthesis of lipids

This gene is a member of the following regulons

• FapR regulon

Gene

Coordinates on the chromosome (coding sequence): 1,208,222 -> 1,209,160

Phenotypes of a mutant

• significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species PubMed

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO₂ (according to Swiss-Prot)

Protein family

• fabH family (according to Swiss-Prot)

Paralogous protein(s)

• FabHB, one of the two proteins has to be present for viability PubMed

Structure

• 1EBL (FabH from E. coli) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis PubMed

Expression and Regulation

Operon

• fabHA-fabF PubMed

Sigma factor

• SigA PubMed

Regulation

• expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
• inhibited by cerulenin PubMed
• induced upon fatty acid biosynthesis inhibition PubMed
• expression is reduced when SigW is activated (by alkaline shock, polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100) PubMed

Regulatory mechanism

• FapR: transcription repression PubMed

References

Reviews

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Original Publications

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