SubtiBank SubtiBank
fabHA [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

fabHA [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells
locus
BSU11330
pI
5.05
mw
33.00 kDa
function
fatty acid biosynthesis
product
beta-ketoacyl-acyl carrier protein synthase III
essential
no
ec
2.3.1.180
synonyms
yjaX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids]

    • This gene is a member of the following [SW|regulons]

  • [SW|FapR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,208,222 -> 1,209,160

    Phenotypes of a mutant

  • significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species [Pubmed|21542858]

    • The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacyl-[acyl-carrier-protein] + CoA + CO2 (according to Swiss-Prot)

    • Protein family

  • fabH family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [SW|FabHB], one of the two proteins has to be present for viability [Pubmed|17114254]

    • Structure

  • [PDB|1EBL] (FabH from ''E. coli'') [Pubmed|10673437]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis [Pubmed|19820084]

    • Expression and Regulation

    Operon

  • ''[SW|fabHA]-[protein|search|fabF]'' [Pubmed|12737802]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|12737802]

    • Regulation

  • expressed when the cells experience a lack of malonyl-CoA ([protein|search|FapR]) [Pubmed|12737802]
  • inhibited by cerulenin [Pubmed|11325930]
  • induced upon fatty acid biosynthesis inhibition [Pubmed|21383089]
  • expression is reduced when [protein|search|SigW] is activated (by alkaline shock, polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100) [Pubmed|21542858]

    • Regulatory mechanism

  • [protein|search|FapR]: transcription repression [Pubmed|12737802]

    • References

    Reviews

  • 15952903,17919287

    • Original Publications

  • 12737802,17114254,10629181,10673437,19820084,21383089,21542858,25196128