hfq [2020-09-10 09:27:54]

RNA chaperone

Locus: BSU_17340

Isoelectric point: 8.70

Molecular weight: 8.38 kDa

Protein length: 73 aa

Gene length: 222 bp

Function: unknown

Product: RNA chaperone

Essential: no

Synonyms: ymaH

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNA chaperones

Gene

Coordinates: 1,867,373 1,867,594

The protein

Protein family

• hfq family (single member, according to UniProt)

Structure

• 3HSB (complex with an RNA aptamer) PubMed

Expression and Regulation

Operons

• Genes: miaA-hfq
  Description: PubMed

• Genes: ymaF-miaA-hfq
  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

• Genes: hfq
  Description: PubMed

  Regulation

  • expression (mRNA levels) is quite constant during growth in minimal medium PubMed

Biological materials

Mutant

• GP22 (hfq::cat), available in Jörg Stülke's lab
• BKE17340 (hfq::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT
• BKK17340 (hfq::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT

Expression vectors

• pGP2166: expression of hfq-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

LacZ fusion

• pGP460 (in pAC7), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1067 (spc, based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original publications

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