SubtiBank SubtiBank
degQ
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degQ

stimulates production of degradative enzymes and of extracellular poly-gamma-glutamate, stimulates phosphorylation of DegU by DegS, gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C)
Locus
BSU_31720
Isoelectric point
6.54
Molecular weight
5.41 kDa
Protein length
46 aa Sequence Blast
Gene length
141 bp Sequence Blast
Function
regulation of exoenzyme synthesis
Product
pleiotropic regulator
Essential
no
Synonyms
sacQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Control of response regulators/ other
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation

    • Gene

    Coordinates
    3,257,092 3,257,232

    Phenotypes of a mutant

  • loss of poly-gamma-glutamate production PubMed, no synthesis of nonribosomal peptides iturin A and plipastatin PubMed1 PubMed2, reduced synthesis of degradative enzymes PubMed, increased genetic competence PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • stimulates autophosphorylation of DegS PubMed

    • Protein family

  • degQ family (single member, according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    degQ
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • ComA: activation, PubMed, in ComA regulon
  • DegU: activation, PubMed, in DegU regulon

    • Regulation

  • controlled by DegU PubMed

    • Additional information

  • the gene is not expressed in the lab strain 168 due to promoter down mutation in the -10 region (T - 10 ---> C) PubMed
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    Biological materials

    Mutant

  • QB4851 (cat), available in Jörg Stülke's lab
  • BKE31720 (degQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTTCCACACTCCTTT, downstream forward: _UP4_TGAAAAAGACTTGGAAACAA
  • BKK31720 (degQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTTCCACACTCCTTT, downstream forward: _UP4_TGAAAAAGACTTGGAAACAA

    • References

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