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mreBH
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mreBH

cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for LytE activity
Locus
BSU_14470
Isoelectric point
5.24
Molecular weight
35.53 kDa
Protein length
335 aa Sequence Blast
Gene length
1008 bp Sequence Blast
Function
cell shape determation
Product
cell shape-determining protein
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,516,574 1,517,581

The protein

Catalyzed reaction/ biological activity

  • forms ring-shaped filaments in a heterologous system PubMed
  • required for the activity of LytE PubMed
  • Protein family

  • FtsA/MreB family (according to UniProt)
  • Paralogous protein(s)

    Structure

  • 1JCF (MreB from Thermotoga maritima, 52% identity) PubMed
  • Localization

  • during logarithmic growth, MreBH forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by MreBH PubMed seem to be misinterpretation of data PubMed
  • normal localization depends on the presence of glucolipids, MreB forms irregular clusters in an ugtP mutant PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigI: sigma factor, PubMed, in SigI regulon
  • Regulatory mechanism

  • WalR: activation, PubMed, in WalR regulon
  • Regulation

  • expression is increased in an ugtP mutant PubMed
  • induced at high temperature (SigI, WalR) PubMed
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    Biological materials

    Mutant

  • BKE14470 (mreBH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCACCCAATCTCT, downstream forward: _UP4_TAGTCTTTACCCGCAAAATT
  • BKK14470 (mreBH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCACCCAATCTCT, downstream forward: _UP4_TAGTCTTTACCCGCAAAATT
  • References

    Localization

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    Other original publications

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