yteR [2019-01-14 09:26:03]

galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX

locus: BSU30120

pI: 5.87

mw: 42.81 kDa

protein length: 373 aa

gene length: 1119 bp

function: intracellular degradation of disaccharides generated by YesX

product: galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

Gene

Coordinates: 3,081,131 → 3,082,243

The protein

Protein family

• glycosyl hydrolase 105 family (according to Swiss-Prot)

Structure

• [PDB|2GH4], [PDB|2D8L] (complex with D-GlcA-Gal-nc)

Expression and Regulation

Operons

• genes: [gene|4ED1DD17694A06CFDFF48943BAE8AEC2CCCB0401|yteP]-[gene|40787292458E5C6676F9F4F6F34C0D6BD5313DE1|yteR]-[gene|1482FD92CB6DC966263F08AD109769728FC708E4|yteS]-[gene|484D17480DFAD3667E0EBC6D38854A7546A8DB46|yteT]-[gene|103A912E971E5760DBA45CC0681D77CED61D1FED|yteU]-[gene|FCAE9513D764B7A9AAB9C59E6CE1D65C400D4B44|yteV]
  description: [Pubmed|22383849]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• MGNA-A811 (yteR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/811 NBRP B. subtilis, Japan]
• BKE30120 (Δ[gene|40787292458E5C6676F9F4F6F34C0D6BD5313DE1|yteR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATCCATTGATCCCATCC, downstream forward: _UP4_CCTCTTTTTAGGTCAGCGGG
• BKK30120 (Δ[gene|40787292458E5C6676F9F4F6F34C0D6BD5313DE1|yteR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATCCATTGATCCCATCC, downstream forward: _UP4_CCTCTTTTTAGGTCAGCGGG

References

• 12884008,16870154,16781735,17449691