ctc

general stress protein, similar to ribosomal protein L25

locus: BSU_00520

pI: 4.22

mw: 21.91 kDa

protein length: 204 aa

gene length: 615 bp

function: [SW|translation] (under stress conditions)

product: ribosomal protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 58,783 59,397

The protein

Protein family

• Bacterial ribosomal protein bL25 family (single member, according to UniProt)

Structure

• [PDB|1FEU] (from ''Thermus thermophilus'', 32% identity, 53% similarity) [Pubmed|11418764]

[SW|Localization]

• in the ribosome (large subunit) [Pubmed|12432960]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|8522540], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

• genes: [gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]-[gene|FE7859E98F26E532AE02CD71B42B845BADBCF06E|spoVC]-[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced under stress conditions ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [pubmed|15805528]

• genes: [gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced under stress conditions ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [pubmed|15805528]

Biological materials

Mutant

• MGNA-B912 (ctc::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1911 NBRP B. subtilis, Japan]
• GP500(spc), available in [SW|Jörg Stülke]'s lab [Pubmed|12432960]
• CS206 (''[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE00520 ([gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCACCATCCTCT, downstream forward: _UP4_TAATAGCTTAAGGCGTAACC
• BKK00520 ([gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCACCATCCTCT, downstream forward: _UP4_TAATAGCTTAAGGCGTAACC

Expression vectors

• expression/ purification with N-terminal His-tag in ''E. coli'': pGP804 (in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab [Pubmed|12432960]

Antibody

• available in [SW|Jörg Stülke]'s lab [Pubmed|12432960]

References

Reviews

• 19216708

Original publications

• 2836704,3123466,3100810,8522540,15805528,12432960,6790515,2836704,116131,11418764,23002217,27197833