yukC

membrane pseudokinase-like protein, part of the type VII protein secretion system YukD-YukC-YukB-YueB-YueC

Locus: BSU_31890

Isoelectric point: 4.68

Molecular weight: 52.00 kDa

Protein length: 451 aa

Gene length: 1356 bp

Function: export of YukE

Product: membrane pseudokinase-like protein, part of the type VII protein secretion system

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,274,462 3,275,817

Phenotypes of a mutant

• loss of secretion of YukE PubMed

The protein

Protein family

• EssB family (single member, according to UniProt)

Structure

• 4ANO (from Geobacillus thermodenitrificans, corresponds to the cytoplasmic part, aa 2 ... 218, 44% identity) PubMed
• 2YNQ (from Geobacillus thermodenitrificans, corresponds to the extracellular part, aa 243 ... 384, 40% identity) PubMed

Localization

• cell membrane (according to UniProt) PubMed

Expression and Regulation

Operons

• Genes: yukE-yukD-yukC-yukB-yueB-yueC-yueD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon

  Regulation

  • expressed in the stationary phase PubMed

Biological materials

Mutant

• MGNA-A626 (yukC::erm), available at the NBRP B. subtilis, Japan
• BKE31890 (yukC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC
• BKK31890 (yukC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACCTGACATTCAACCAGATC, downstream forward: _UP4_TAACGTGAGACATGAAGCGC

References

Reviews

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Original publications

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