nasB

nitrate reductase (electron transfer subunit)

Locus: BSU_03320

Isoelectric point: 5.48

Molecular weight: 84.57 kDa

Protein length: 771 aa

Gene length: 2316 bp

Function: utilization of nitrate

Product: nitrate reductase (electron transfer subunit)

Essential: no

Synonyms: nasBA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of nitrate/ nitrite

Gene

Coordinates: 360,442 362,757

The protein

Paralogous protein(s)

• NasD

Cofactors

• FAD PubMed

Structure

• 3KLJ (from Clostridium acetobutylicum, corresponds to aa 2 ... 371, 27% identity) PubMed

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • TnrA: activation, PubMed, in TnrA regulon
  • NsrR: repression, PubMed, in NsrR regulon
  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • nasD: expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• 1A971 ( nasB::erm), PubMed, available at BGSC
• BKE03320 (nasB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATCAGACCTCCTTTG, downstream forward: _UP4_TGAGGCATTTTGACTGAACG
• BKK03320 (nasB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATCAGACCTCCTTTG, downstream forward: _UP4_TGAGGCATTTTGACTGAACG

References

Reviews

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Original publications

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