SubtiBank SubtiBank
queG
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queG

epoxyqueuosine reductase
Locus
BSU_08910
Isoelectric point
7.35
Molecular weight
48.37 kDa
Protein length
386 aa Sequence Blast
Gene length
1161 bp Sequence Blast
Function
synthesis of queuosine, tRNA modification
Product
epoxyqueuosine reductase
Essential
no
Synonyms
ygaP,yhbA

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
967,935 969,095

The protein

Catalyzed reaction/ biological activity

  • conversion of epoxyqueuosine to queuosine PubMed
  • AH2 + epoxyqueuosine34 in tRNA = A + H2O + queuosine34 in tRNA (according to UniProt)
  • Protein family

  • QueG family (single member, according to UniProt)
  • Domains

  • 4Fe-4S ferredoxin-type domain (aa 178-208) (according to UniProt)
  • Cofactors

  • cobalamin
  • Fe-S cluster PubMed
  • Structure

    Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-A241 (yhbA::erm), available at the NBRP B. subtilis, Japan
  • BKE08910 (queG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT, downstream forward: _UP4_TGACATACGTATAGAGCAGA
  • BKK08910 (queG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTGCACAGCCTCCT, downstream forward: _UP4_TGACATACGTATAGAGCAGA
  • References

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