yugF

similar to dihydrolipoamide S-acetyltransferase

locus: BSU_31420

pI: 8.36

mw: 31.18 kDa

protein length: 273 aa

gene length: 822 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,227,581 3,228,402

The protein

Protein family

• dmpD/todF/xylF esterase family (single member, according to UniProt)

[SW|Domains]

• [SW|AB hydrolase-1 domain] (aa 29-131) (according to UniProt)

Structure

• [PDB|5JZ9] (26% identity) [pubmed|28380256]

Expression and Regulation

Operons

• genes: [gene|3EE40BA7FB4951A72964F847B6A678B48579D191|yugF]
  description: [Pubmed|9274030]

  regulation

  • expressed during [category|SW 4.2|Sporulation] [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B550 (yugF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1549 NBRP B. subtilis, Japan]
• BKE31420 ([gene|3EE40BA7FB4951A72964F847B6A678B48579D191|yugF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTGTCACTCCCTTT, downstream forward: _UP4_TAACAAAAAAACCGGTGCTG
• BKK31420 ([gene|3EE40BA7FB4951A72964F847B6A678B48579D191|yugF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTGTCACTCCCTTT, downstream forward: _UP4_TAACAAAAAAACCGGTGCTG

References

• 9274030,28380256