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rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]
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rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]

RNase J1
Locus
BSU14530
Isoelectric point
5.00
Molecular weight
61.00 kDa
Function
RNA processing
Product
RNase J1
Essential
no
Synonyms
ykqC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
  • Groups of genes → Essential genes

    • Categories containing this gene/protein

  • RNAses, essential genes

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,523,118 -> 1,524,785

    Phenotypes of a mutant

  • the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
  • it is not possible to construct a rnjA rny double mutant PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades SR5 and gapA RNAs 27449348

    • Protein family

  • RNase J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • RnjB

    • Effectors of protein activity

  • initeraction with GapA-YkzW stimulates activity PubMed

    • Structure

  • 3ZQ4 PubMed

    • Localization

  • cytoplasm, colocalizes with the ribosomes PubMed

    • Interactions

  • part of the RNA degradosome PubMed
  • RnjA-RnjB PubMed
  • RnjA-PfkA PubMed
  • RnjA-PnpA PubMed, K(D) of the interaction: 250 nM PubMed
  • RnjA-Rny PubMed
  • RnjA-CshA PubMed
  • GapA-RnjA PubMed, about 1% of all GapA molecules participate in this interaction PubMed, this interaction is stabilized in the presence of YkzW PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • required for thrS RNA processing, involved in maturation of the 5’-end of the16S rRNA

    • Expression and Regulation

    Operon

  • rpoY-rnjA PubMed

    • Sigma factor

  • SigA PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of [rnjA|search|RNase J1] PubMed

    • Biological materials

    Mutant

  • GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
  • SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
  • GP2506 (rnjA::spc), available in Jörg Stülke's lab

    • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab

    • LacZ fusion

  • pGP418 (in pAC7), available in Jörg Stülke's lab

    • GFP fusion

  • GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1075 (aphA3), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Harald Putzer, IBPC Paris, France Homepage
  • David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
  • Ciaran Condon, IBPC, Paris, France Homepage

    • References

    Reviews

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    Original publications

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