SubtiBank SubtiBank
rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]

RNase J1
Locus
BSU14530
Isoelectric point
5.00
Molecular weight
61.00 kDa
Function
RNA processing
Product
RNase J1
Essential
no
Synonyms
ykqC

Genomic Context

      
Loading

Categories containing this gene/protein

Categories containing this gene/protein

Gene

Coordinates on the chromosome (coding sequence)
1,523,118 -> 1,524,785

Phenotypes of a mutant

  • the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
  • it is not possible to construct a rnjA rny double mutant PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades SR5 and gapA RNAs 27449348
  • Protein family

  • RNase J subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

    Effectors of protein activity

  • initeraction with GapA-YkzW stimulates activity PubMed
  • Structure

    Localization

  • cytoplasm, colocalizes with the ribosomes PubMed
  • Interactions

  • part of the RNA degradosome PubMed
  • RnjA-RnjB PubMed
  • RnjA-PfkA PubMed
  • RnjA-PnpA PubMed, K(D) of the interaction: 250 nM PubMed
  • RnjA-Rny PubMed
  • RnjA-CshA PubMed
  • GapA-RnjA PubMed, about 1% of all GapA molecules participate in this interaction PubMed, this interaction is stabilized in the presence of YkzW PubMed
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • required for thrS RNA processing, involved in maturation of the 5’-end of the16S rRNA
  • Expression and Regulation

    Operon

    Sigma factor

    Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of [rnjA|search|RNase J1] PubMed
  • Biological materials

    Mutant

  • GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
  • SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
  • GP2506 (rnjA::spc), available in Jörg Stülke's lab
  • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
  • LacZ fusion

  • pGP418 (in pAC7), available in Jörg Stülke's lab
  • GFP fusion

  • GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1075 (aphA3), available in Jörg Stülke's lab
  • Labs working on this gene/protein

    References

    Reviews

    Loading

    Original publications

    Loading