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rnjA [2017-02-27 15:02:04]
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rnjA [2017-02-27 15:02:04]

RNase J1
Locus
BSU14530
Isoelectric point
5.00
Molecular weight
61.00 kDa
Protein length
555 aa Sequence Blast
Gene length
1665 bp Sequence Blast
Function
RNA processing
Product
RNase J1
Essential
no
Synonyms
ykqC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates on the chromosome (coding sequence)
1,523,118 -> 1,524,785

Phenotypes of a mutant

  • the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
  • it is not possible to construct a rnjA rny double mutant PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades SR5 and gapA RNAs 27449348
  • Protein family

  • RNase J subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

    Effectors of protein activity

  • initeraction with GapA-Sr1 stimulates activity PubMed
  • Structure

    Localization

  • cytoplasm, colocalizes with the ribosomes PubMed
  • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • required for thrS RNA processing, involved in maturation of the 5’-end of the16S rRNA
  • Expression and Regulation
    [[this]]

    Biological materials

    Mutant

  • GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
  • SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
  • GP2506 (rnjA::spc), available in Jörg Stülke's lab
  • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
  • pGP1441: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
  • LacZ fusion

  • pGP418 (in pAC7), available in Jörg Stülke's lab
  • GFP fusion

  • GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
  • GP1722 (in pBP43), expression of rnjA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

  • GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1075 (aphA3), available in Jörg Stülke's lab
  • Labs working on this gene/protein

    References

    Reviews

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    Original publications

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