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gerD [Wed Jan 06 2016 13:28:29 GMT+0100 (CET)]
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gerD [Wed Jan 06 2016 13:28:29 GMT+0100 (CET)]

scaffold of the germinosome, required for clustering of germinant receptors in the spore inner membrane
locus
BSU01550
pI
4.00
mw
20.00 kDa
function
germination
product
lipoprotein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|germination], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigF regulon], [SW|SigG regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    158,515 -> 159,072

    Phenotypes of a mutant

  • defective in [SW|germination] in response to nutrients [Pubmed|24530795]

    • The protein

    Catalyzed reaction/ biological activity

  • required for clustering of germinant receptors in the spore inner membrane [Pubmed|21696470]

    • [SW|Domains]

  • has an N-terminal signal sequence and a signal for fixation to lipid [Pubmed|23335419]

    • Structure

  • [PDB|4O8W] [Pubmed|24530795]

    • [SW|Localization]

  • outer surface of the inner spore membrane [Pubmed|23335419,21696470]
  • inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction [Pubmed|19332816]

    • [SW|Interactions]

  • forms trimeric rope-like structures [Pubmed|24530795]
  • [protein|search|GerD] is part of the [SW|germinosome] in the spore inner membrane [Pubmed|21696470]
  • [protein|search|GerD]-[protein|search|Lgt] to fix [protein|search|GerD] to diacylglycerol in the membrane [Pubmed|22493018]

    • Additional information

  • 3,500 molecules are present per spore [Pubmed|23749970]

    • Expression and Regulation

    Operon

  • [protein|search|gerD] [Pubmed|2517635]

    • [SW|Sigma factor]

  • [protein|search|SigF] [Pubmed|15699190], [protein|search|SigG] [Pubmed|1906867,15699190]

    • Regulation

  • expression level depends on sporulation conditions (medim composition, temperature) [Pubmed|22327596]

    • Additional information

  • 3,500 molecules are present per spore [PubMed|23749970]

    • Biological materials

    Mutant

  • 1A677 ( ''gerD''::''erm''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A677&Search=1A677 BGSC]
  • 1G17 ( ''gerD''::''spec''), [Pubmed|15126458], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1G17&Search=1G17 BGSC]

    • Antibody

  • available in [SW|Anne Moir] lab

    • Labs working on this gene/protein

  • [SW|Anne Moir], University of Sheffield, UK

    • References

  • 1906867,19332816,17122337,21725007,18556788,19332816,2517635,21696470,22327596,22493018,23335419,23749970,21398556,24530795,24752279