gerD

scaffold of the germinosome, required for clustering of germinant receptors in the spore inner membrane

Locus: BSU_01550

Isoelectric point: 4.86

Molecular weight: 20.97 kDa

Protein length: 185 aa

Gene length: 558 bp

Function: germination

Product: lipoprotein

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Sporulation → Germination → Additional germination proteins
• Groups of genes → Membrane proteins

Gene

Coordinates: 158,515 159,072

Phenotypes of a mutant

• defective in germination in response to nutrients PubMed

The protein

Catalyzed reaction/ biological activity

• required for clustering of germinant receptors in the spore inner membrane PubMed

Domains

• has an N-terminal signal sequence and a signal for fixation to lipid PubMed

Structure

• 4O8W PubMed

Localization

• outer surface of the inner spore membrane PubMed
• inner spore membrane, spore coat, cortex, germ cell wall, some GerD is also present in the soluble fraction PubMed

Additional information

• 3,500 molecules are present per spore PubMed

Expression and Regulation

Operons

• Genes: gerD
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expression level depends on sporulation conditions (medim composition, temperature) PubMed

  Additional information

  • 3,500 molecules are present per spore PubMed

Biological materials

Mutant

• 1A677 ( gerD::erm), [Pubmed| ], available at BGSC
• 1G17 ( gerD::spec), PubMed, available at BGSC
• BKE01550 (gerD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTAAGCTCCTTTCAC, downstream forward: _UP4_TAAAGGGAAAGCCGGGATCT
• BKK01550 (gerD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTTAAGCTCCTTTCAC, downstream forward: _UP4_TAAAGGGAAAGCCGGGATCT

Antibody

• available in Anne Moir lab

Labs working on this gene/protein

• Anne Moir, University of Sheffield, UK

References

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