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pta [2018-10-04 09:41:45]
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pta [2018-10-04 09:41:45]

phosphotransacetylase
locus
BSU37660
pI
4.65
mw
34.64 kDa
protein length
323 aa Sequence Blast
gene length
969 bp Sequence Blast
function
overflow metabolism
product
phosphotransacetylase
essential
no
ec
2.3.1.8
synonyms
ipa-88d,ywfJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    3,865,355 → 3,866,326

    The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)

    • Protein family

  • phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

    • Modification

  • phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) [Pubmed|17218307]

    • Structure

  • [PDB|1XCO] (complex with acetylphosphate)[pubmed|16283428]
  • [PDB|1TD9]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]
    description
    [Pubmed|10423526]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10423526], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|12850135,10559153], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expression activated by glucose (2.6 fold) (CcpA) [Pubmed|12850135,10559153]
    • view in new tab

    Biological materials

    Mutant

  • GP668 (aphA3), available in [SW|Jörg Stülke]'s lab
  • BKE37660 (Δ[gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG
  • BKK37660 (Δ[gene|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 15755952,26098117,16283428,30265683