pta [2019-08-12 12:07:44]

phosphotransacetylase

Locus: BSU_37660

Isoelectric point: 4.65

Molecular weight: 34.64 kDa

Protein length: 323 aa

Gene length: 972 bp

Function: overflow metabolism

Product: phosphotransacetylase

Essential: no

E.C.: 2.3.1.8

Synonyms: ipa-88d,ywfJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 3,865,355 3,866,326

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)

Protein family

• phosphate acetyltransferase and butyryltransferase family (with Ptb, according to UniProt)

Modification

• phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed

Structure

• 1XCO (complex with acetylphosphate)PubMed
• 1TD9

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: pta
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: activation, PubMed, in CcpA regulon

  Regulation

  • expression activated by glucose (2.6 fold) (CcpA) PubMed

Biological materials

Mutant

• GP668 (aphA3), available in Jörg Stülke's lab
• BKE37660 (pta::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG
• BKK37660 (pta::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAATAAAACCTCCTCAAA, downstream forward: _UP4_TAATAAAATTGAAGACAATG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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