pta [Sun Nov 27 2016 18:12:14 GMT+0100 (CET)]

phosphotransacetylase

locus: BSU37660

pI: 4.00

mw: 34.00 kDa

function: overflow metabolism

product: phosphotransacetylase

essential: no

ec: 2.3.1.8

synonyms: ipa-88d, ywfJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

[SW|Categories] containing this gene/protein

• [SW|carbon core metabolism], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,865,355 -> 3,866,326

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA phosphate = CoA acetyl phosphate (according to Swiss-Prot)

Protein family

• phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

Modification

• phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) [Pubmed|17218307]

Structure

• [PDB|1XCO] (complex with acetylphosphate), [PDB|1TD9]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''pta'' [Pubmed|10423526]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|10423526]

Regulation

• expression activated by glucose (2.6 fold) (CcpA) [Pubmed|12850135,10559153]

Regulatory mechanism

• [protein|search|CcpA]: transcription activation [Pubmed|12850135,10559153]

Biological materials

Mutant

• GP668 (aphA3), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 15755952,26098117