thrS

threonyl-tRNA synthetase (major)

locus: BSU_28950

pI: 5.21

mw: 73.34 kDa

protein length: 643 aa

gene length: 1932 bp

function: translation

product: threonyl-tRNA synthetase (major)

essential: no

ec: 6.1.1.3

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]

Gene

Coordinates: 2,959,257 2,961,188

The protein

Catalyzed reaction/ biological activity

• ATP + L-threonine + tRNA^Thr --> AMP + diphosphate + H⁺ + L-threonyl-tRNA^Thr (according to UniProt)

Protein family

• [SW|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)

Paralogous protein(s)

• [protein|CA74CB721842927F67FBC3B5C27D2EE46B30CE1C|ThrZ]:

[SW|Domains]

• [SW|TGS domain] (aa 3-64) (according to UniProt)

Modification

• Cys573 is S-bacillithiolated by NaOCl stress [Pubmed|22938038]

Structure

• [PDB|1TJE] (from ''Escherichia coli'', 44% identity, 63% similarity) [Pubmed|15525511]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS]
  description: [Pubmed|8969504]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8288542], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: anti-termination, in [regulon|T-box|T-box]

  regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

• genes: [gene|2AEF6D56D884CA6A0BD50FABD27F405DC2B506C7|dnaB]-[gene|AC1B507C6B1640E9BEA1F9F01628120F3680C976|dnaI]-[gene|72A4458679EA1451FA3B63D44C3BE93ACA8B397F|ytxB]-[gene|3EE0D03469D893515CAA4961BF923BE3A6769535|ytxC]-[gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE28950 ([gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGTCACTCCTTTTT, downstream forward: _UP4_TAAAATAAAAAAGCATGATC
• BKK28950 ([gene|3E1131CFA3EDEB3865638F09BE2F6B6ED2570BE2|thrS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGTCACTCCTTTTT, downstream forward: _UP4_TAAAATAAAAAAGCATGATC

References

• 22938038,8288542,1379177,19258532,7476165,12136084,8969504,17114254,22848659,15378759,29794222