SubtiBank SubtiBank
mmgD
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mmgD

2-methylcitrate synthase
Locus
BSU_24140
Isoelectric point
6.20
Molecular weight
41.95 kDa
Protein length
372 aa Sequence Blast
Gene length
1119 bp Sequence Blast
Function
mother cell metabolism
Product
2-methylcitrate synthase
Essential
no
E.C.
2.3.3.5
Synonyms
yqiO,prpC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,509,654 2,510,772

The protein

Catalyzed reaction/ biological activity

  • Propanoyl-CoA + H2O + oxaloacetate --> 2-methylcitrate + CoA PubMed
  • does also have (albeit weaker) citrate synthase activity PubMed
  • acetyl-CoA + H2O + oxaloacetate --> citrate + CoA + H+ (according to UniProt)
  • Protein family

  • citrate synthase family (with CitA and CitZ, according to UniProt)
  • Paralogous protein(s)

    Structure

  • 3HWK (from Mycobacterium tuberculosis, 38% identity) PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed
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    Biological materials

    Mutant

  • BKE24140 (mmgD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
  • BKK24140 (mmgD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
  • References

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