mmgD

2-methylcitrate synthase

Locus: BSU_24140

Isoelectric point: 6.20

Molecular weight: 41.95 kDa

Protein length: 372 aa

Gene length: 1119 bp

Function: mother cell metabolism

Product: 2-methylcitrate synthase

Essential: no

E.C.: 2.3.3.5

Synonyms: yqiO,prpC

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 2,509,654 2,510,772

The protein

Catalyzed reaction/ biological activity

• Propanoyl-CoA + H₂O + oxaloacetate --> 2-methylcitrate + CoA PubMed
• does also have (albeit weaker) citrate synthase activity PubMed
• acetyl-CoA + H₂O + oxaloacetate --> citrate + CoA + H⁺ (according to UniProt)

Protein family

• citrate synthase family (with CitA and CitZ, according to UniProt)

Paralogous protein(s)

• CitZ, CitA

Structure

• 3HWK (from Mycobacterium tuberculosis, 38% identity) PubMed

Expression and Regulation

Operons

• Genes: mmgA-mmgB-mmgC-mmgD-mmgE-mmgF
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
  • strongly expressed during oligotrophic growth PubMed

Biological materials

Mutant

• BKE24140 (mmgD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT
• BKK24140 (mmgD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCTCAACCTCCGTTT, downstream forward: _UP4_AAATCATGAAAGGAGCTGGT

References

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