fatR [2019-01-04 13:25:08]

transcriptional repressor of the [gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ] operon

locus: BSU27170

pI: 5.62

mw: 22.07 kDa

protein length: 194 aa

gene length: 582 bp

function: unknown

product: transcriptional repressor of the [gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ] operon ([SW|TetR family])

essential: no

synonyms: yrhI,bscR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 2,777,070 → 2,777,654

The protein

Modification

• phosphorylated by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA] on Tyr-45, this results in displacement of FatR from the DNA [Pubmed|23939619]

Structure

• [PDB|4ME9] (from B. cereus, 38% identity)

Expression and Regulation

Operons

• genes: [gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
  description: [Pubmed|10917605]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421,12775685], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|9636707], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|3DA3FFB295F5513A220CB163994EE0F356C810E6|FatR]: repression, [Pubmed|11734890], in [regulon|3DA3FFB295F5513A220CB163994EE0F356C810E6|FatR regulon]

  regulation

  • expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

  additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]

• genes: [gene|6D5089931FBDB398A7FAF3C8DDF341EB4949AEFE|yrhH]-[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
  description: [Pubmed|18179421]

  regulation

  • Expressed under stress conditions ([protein|search|SigW], [protein|search|SigX]) [Pubmed|9683469]

  additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]

Biological materials

Mutant

• MGNA-A177 (yrhI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/177 NBRP B. subtilis, Japan]
• BKE27170 (Δ[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCTGACAACCTCC, downstream forward: _UP4_CAAAAATAGGAAAGGGAGAT
• BKK27170 (Δ[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAATCTGACAACCTCC, downstream forward: _UP4_CAAAAATAGGAAAGGGAGAT

References

• 11734890,11574077,11377867,10917605,12207695,9636707,23939619,21926231