polA [2017-8-11 16:0:3]

DNA polymerase I, required for repair of UV- or MMS-induced DNA damage

locus: BSU29090

pI: 4.92

mw: 98.89 kDa

protein length: 880 aa

gene length: 2640 bp

function: DNA replication

product: DNA polymerase I

essential: no

ec: 2.7.7.7

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,973,182 → 2,975,824

Phenotypes of a mutant

• strongly reduced stationary phase mutagenesis [Pubmed|27399782]

The protein

Catalyzed reaction/ biological activity

• has DNA polymerase and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity [Pubmed|16045613]

Protein family

• DNA polymerase type-A family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|ExoR], one of the two proteins has to be present for viability [Pubmed|17114254]

Structure

• [PDB|1XWL] (large fragment, corresponds to AA 310 to 880, Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
  description: [pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

References

• 17114254,16045613,17905985,27399782