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defA [2019-08-01 11:07:39]
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defA [2019-08-01 11:07:39]

formylmethionine deformylase
Locus
BSU_15720
Isoelectric point
4.73
Molecular weight
17.63 kDa
Protein length
160 aa Sequence Blast
Gene length
483 bp Sequence Blast
Function
translation
Product
formylmethionine deformylase
Essential
no
E.C.
3.5.1.88
Synonyms
yloK,def
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other
  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein maturation

    • Gene

    Coordinates
    1,646,512 1,646,994

    Phenotypes of a mutant

  • a defA defB double mutant is not viable unless the cells acquire suppressor mutations in fmt, glyA or folD PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Formyl-L-methionyl peptide + H2O = formate + methionyl peptide (according to Swiss-Prot)

    • Protein family

  • polypeptide deformylase family (with DefB, according to UniProt)

    • Paralogous protein(s)

  • DefB

    • Structure

  • 1WS0 (from B.cereus, 67% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    defA-fmt-yloM-yloN
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Genes
    yloC-remA-gmk-yloH-yloI-priA-defA-fmt-yloM-yloN

    Description

  • PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE15720 (defA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG
  • BKK15720 (defA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG

    • References

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