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defA
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defA

formylmethionine deformylase
locus
BSU_15720
pI
4.73
mw
17.63 kDa
protein length
160 aa Sequence Blast
gene length
483 bp Sequence Blast
function
translation
product
formylmethionine deformylase
essential
no
ec
3.5.1.88
synonyms
yloK,def
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.1|Protein maturation]

    • Gene

    Coordinates
    1,646,512 1,646,994

    Phenotypes of a mutant

  • a ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant is not viable unless the cells acquire suppressor mutations in ''[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt], [gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]'' or ''[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]'' [Pubmed|27983482]

    • The protein

    Catalyzed reaction/ biological activity

  • H2O + N-terminal N-formyl-L-methionyl-[peptide] --> formate + N-terminal L-methionyl-[peptide] (according to UniProt)

    • Protein family

  • polypeptide deformylase family (with [protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|DefB], according to UniProt)

    • Paralogous protein(s)

  • [protein|53B760316B19E142FC3ECAD2CFAA540377B08BBC|DefB]

    • Structure

  • [PDB|1WS0] (from B.cereus, 67% identity) [pubmed|16049914]

    • Expression and Regulation

    Operons

    genes
    [gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
    description
    [Pubmed|16964327]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16964327], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    genes
    [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

    description

  • [pubmed|22383849]
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    Biological materials

    Mutant

  • BKE15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG
  • BKK15720 ([gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTGTTACCCTCCAAGA, downstream forward: _UP4_CTAGCGGATATGGAAGGATG

    • References

  • 11429456,12627383,18288106,16964327,23770820,27983482,16049914