ypuI [2019-06-07 11:15:27]

rRNA pseudouridine 2633 synthase

locus: BSU23200

pI: 5.49

mw: 20.21 kDa

protein length: 179 aa

gene length: 540 bp

function: translation

product: rRNA pseudouridine 2633 synthase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 2,424,654 2,425,193

The protein

[SW|Localization]

• forespore outer membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
  description: [Pubmed|7934830]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• MGNA-A094 (ypuI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/94 NBRP B. subtilis, Japan]
• BKE23200 ([gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCTTCTTCCTTAT, downstream forward: _UP4_TAACGTTTACTCTCCCTTTT
• BKK23200 ([gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCTTCTTCCTTAT, downstream forward: _UP4_TAACGTTTACTCTCCCTTTT

References

• 7934830