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nfo [2019-07-05 10:57:42]
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nfo [2019-07-05 10:57:42]

type IV apurinic/apyrimidinic endonuclease
locus
BSU25130
pI
5.37
mw
32.92 kDa
protein length
297 aa Sequence Blast
gene length
894 bp Sequence Blast
function
repair of oxidative DNA damage in spores
product
type IV apurinic/apyrimidinic endonuclease
essential
no
synonyms
yqfS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.2|A/P endonucleases]

    • Gene

    Coordinates
    2,593,281 2,594,174

    Phenotypes of a mutant

  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]'' [Pubmed|24244006]
  • an ''[gene|FCDE5EA6E50A8C72A499E2F1FFB808DC86466A29|exoR] [gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]'' double mutant is sensitive to radiation [Pubmed|24123749]
  • sensitive to blue light-induced DNA damage [pubmed|30054368]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)

    • Protein family

  • AP endonuclease 2 family (according to Swiss-Prot)

    • Structure

  • [PDB|3AAL] (from Geobacillus kaustophilus, 80% identity) [pubmed|21358045]

    • Expression and Regulation

    Operons

    genes
    [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C462 (yqfS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2460 NBRP B. subtilis, Japan]
  • available in [SW|Mario Pedraza-Reyes]'s lab
  • GP899 (''nfo''::''kan'') and GP1502 (''nfo''::''cat''), available in [SW|Jörg Stülke]'s lab
  • BKE25130 ([gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC
  • BKK25130 ([gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCCTATTCTCAGCAACAGGT, downstream forward: _UP4_TAAAAAATGGGGGATAACAC

    • References

    Reviews

  • 22933559

    • Original publications

  • 24244006,18203828,16237020,12949090,19930460,12486072,21441501,24123749,24123749,24914186,21358045,30054368