cysC [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

adenylyl-sulfate kinase

locus: BSU15600

pI: 5.08

mw: 22.00 kDa

function: sulfate reduction and activation

product: adenylyl-sulfate kinase

essential: no

ec: 2.7.1.25

synonyms: ylnC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]

[SW|Categories] containing this gene/protein

• [SW|sulfur metabolism]

This gene is a member of the following [SW|regulons]

• [SW|CymR regulon], [SW|S-box]

Gene

Coordinates on the chromosome (coding sequence): 1,633,369 -> 1,633,962

The protein

Catalyzed reaction/ biological activity

• ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate (according to Swiss-Prot)

Protein family

• APS kinase family (according to Swiss-Prot)

Structure

• [PDB|3CR8] (from ''Thiobacillus denitrificans'', 46% identity, 62% similarity) [Pubmed|19770499]

Expression and Regulation

Operon

• ''[protein|search|cysH]-[protein|search|cysP]-[protein|search|sat]-[protein|search|cysC]-[protein|search|ylnD]-[protein|search|ylnE]-[protein|search|ylnF]'' [Pubmed|11004190]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|11004190]

Regulation

• repressed by casamino acids [Pubmed|12107147]
• repressed in the presence of cysteine ([protein|search|CymR])
• induced by methionine starvation ([protein|search|S-box]) [Pubmed|10094622]

Regulatory mechanism

• [protein|search|S-box]: transcription termination/ antitermination, the [protein|search|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622]
• [protein|search|CymR]: transcription repression

Labs working on this gene/protein

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

• 16267287,11004190,12107147,18039762