cysC

adenylyl-sulfate kinase

Locus: BSU_15600

Isoelectric point: 5.08

Molecular weight: 22.40 kDa

Protein length: 197 aa

Gene length: 594 bp

Function: sulfate reduction and activation

Product: adenylyl-sulfate kinase

Essential: no

E.C.: 2.7.1.25

Synonyms: ylnC

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Sulfur metabolism
• Metabolism → Additional metabolic pathways → Sulfur metabolism → sulfur metabolism/ general

Gene

Coordinates: 1,633,369 1,633,962

The protein

Catalyzed reaction/ biological activity

• adenosine 5'-phosphosulfate + ATP --> 3'-phosphoadenylyl sulfate + ADP + H⁺ (according to UniProt)

Protein family

• APS kinase family (with YisZ, according to UniProt)

Structure

• 3CR8 (from Thiobacillus denitrificans, 46% identity, 62% similarity) PubMed

Expression and Regulation

Operons

• Genes: cysH-cysP-sat-cysC-ylnD-sirB-ylnF
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CymR: repression, in CymR regulon
  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• MGNA-B367 (ylnC::erm), available at the NBRP B. subtilis, Japan
• BKE15600 (cysC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCGATTTGTCACTGCCAGTC, downstream forward: _UP4_TGAATATGATCTGCTGCGTT
• BKK15600 (cysC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCGATTTGTCACTGCCAGTC, downstream forward: _UP4_TGAATATGATCTGCTGCGTT

Labs working on this gene/protein

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

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