srfAB

surfactin synthetase / competence

Locus: BSU_03490

Isoelectric point: 4.90

Molecular weight: 400.86 kDa

Protein length: 3583 aa

Gene length: 10752 bp

Function: antibiotic synthesis

Product: surfactin synthetase / competence

Essential: no

Synonyms: comL

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of antibacterial compounds
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming
• Lifestyles → Coping with stress → Biosynthesis of antibacterial compounds
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 387,744 398,495

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Paralogous protein(s)

• partial match (more than 1,000 amino acids): DhbF, PksJ, PksN, PpsA, PpsB, PpsE, SrfAC

Domains

• 3 Carrier domains (aa 965-1039, aa 2005-2080, aa 3034-3108) (according to UniProt)

Modification

• phosphorylation on Ser-999 AND Ser-2045 PubMed
• phosphorylated on Arg-1378 PubMed

Structure

• 2VSQ (termination module of SrfAC, 39% identity) PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: srfAA-srfAB-comS-srfAC-srfAD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon
  • CodY: repression, PubMed, in CodY regulon
  • ComA: activation, PubMed, in ComA regulon
  • PerR: activation, PubMed, in PerR regulon
  • Spx: repression, PubMed, in Spx regulon
  • Abh: repression, PubMed, in Abh regulon

  Regulation

  • expressed at high cell density (ComA) PubMed

  Additional information

  • A ncRNA is predicted between 'hxlR' and 'srfAA' PubMed

Biological materials

Mutant

• BKE03490 (srfAB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTTTGCTCATATGCCACC, downstream forward: _UP4_AACGAAAGCAAGGAGGAGCA
• BKK03490 (srfAB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTTTTGCTCATATGCCACC, downstream forward: _UP4_AACGAAAGCAAGGAGGAGCA

References

Reviews

Loading

Original publications

Loading