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mntA [2017-10-19 17:42:15]
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mntA [2017-10-19 17:42:15]

manganese ABC transporter (binding protein, lipoprotein)
locus
BSU30770
pI
6.16
mw
33.26 kDa
protein length
306 aa Sequence Blast
gene length
918 bp Sequence Blast
function
manganese uptake
product
manganese ABC transporter (binding protein)
essential
no
synonyms
ytgA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.2|Manganese]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    3,145,038 → 3,145,958

    Phenotypes of a mutant

  • essential according to [http://www.ncbi.nlm.nih.gov/pubmed/17114254 Kobayashi et al.], but a mutant has been constructed and studied by [http://www.ncbi.nlm.nih.gov/pubmed/10760146 Que and Helmann, 2000]

    • The protein

    Protein family

  • bacterial solute-binding protein 9 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|ZnuA]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), extracellular lipoprotein (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]-[gene|7821C82C6C30079F713B84F1E4D654E07CE5072E|mntB]-[gene|1B7C4AD6CD0632051A0E8CA57034872D13FF67F9|mntC]-[gene|F7DA077FE47DBB409BE2B1D9F12A9D719C246166|mntD]
    description
    [Pubmed|10760146]

    regulatory mechanism

  • [protein|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR]: repression, [Pubmed|12950915], in [regulon|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|MntR regulon]

    • regulation

  • repressed at high Mn(II) concentrations ([protein|search|MntR]) [Pubmed|12950915]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mntA]' and '[protein|search|menC]' [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-A285 (ytgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/285 NBRP B. subtilis, Japan]
  • BKE30770 (Δ[gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA
  • BKK30770 (Δ[gene|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|mntA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTTCTCCTCCTCTTTG, downstream forward: _UP4_TAAAGAAAAGAGGTGGAGGA

    • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

    • References

  • 10760146,10092453,12950915,10760146,18957862,10760146,17114254,20525796