SubtiBank SubtiBank
ygaF
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ygaF

similar to thioredoxin-dependent hydroperoxide peroxidase
Locus
BSU_08720
Isoelectric point
5.86
Molecular weight
17.97 kDa
Protein length
157 aa Sequence Blast
Gene length
474 bp Sequence Blast
Function
resistance against oxidative stress
Product
unknown
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
943,891 944,364

The protein

Catalyzed reaction/ biological activity

  • [protein]-dithiol + hydroperoxide --> [protein]-disulfide + alcohol + H2O (according to UniProt)
  • Protein family

  • peroxiredoxin family (according to UniProt)
  • Domains

  • Thioredoxin domain (aa 3-156) (according to UniProt)
  • Structure

  • 5ENU (from Burkholderia ambifaria, 48% identity)
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • BKE08720 (ygaF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTTACCTCCGGATG, downstream forward: _UP4_TAAATCTCTATGAGCCTATG
  • BKK08720 (ygaF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTACGTTACCTCCGGATG, downstream forward: _UP4_TAAATCTCTATGAGCCTATG
  • Expression vector

  • pGP2338: expression of ygaF via pBQ200 in B. subtilis, available in Jörg Stülke's lab
  • References

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