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efeU [2017-11-16 13:22:29]
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efeU [2017-11-16 13:22:29]

elemental iron uptake system (permease), high affinity uptake of ferric iron (Fe(III))
locus
BSU38280
pI
8.96
mw
52.24 kDa
protein length
481 aa Sequence Blast
gene length
1443 bp Sequence Blast
function
elemental iron uptake
product
elemental iron uptake system (permease)
essential
no
synonyms
ywbL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.2|Elemental iron transport system]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.5|Elemental iron transport system]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,929,105 → 3,930,550

    The protein

    [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]-[gene|B31884DC49684A276D65E21A5F59AFDADEB9749C|efeO]-[gene|3985F88334D7B034A66473C5BB39188AB61A65F6|efeB]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23764491], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9683469], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

    • regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B226 (ywbL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1225 NBRP B. subtilis, Japan]
  • BKE38280 (Δ[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGTTCCTCCTATAAT, downstream forward: _UP4_ACAAACGGAAAGGAACTGAC
  • BKK38280 (Δ[gene|3BED98E311EF380AE77327C7F0A83254805089C0|efeU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGTTCCTCCTATAAT, downstream forward: _UP4_ACAAACGGAAAGGAACTGAC

    • References

  • 16672620,23180473,12354229,23764491,27795321