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yxaL [2019-02-19 18:52:47]
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yxaL [2019-02-19 18:52:47]

similar to Ser/Thr kinase, increases the processivity of the PcrA helicase
locus
BSU39940
pI
6.38
mw
43.72 kDa
protein length
410 aa Sequence Blast
gene length
1233 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
yxaK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    4,102,429 4,103,661

    The protein

    Structure

  • [PDB|3HXJ] (from Methanococcus maripaludis, 33% Identity)

    • [SW|Localization]

  • extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|0649520164CCC6120C40CE15EF08994BE0E79A25|yxaJ]-[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
    description
    [Pubmed|10746760]

    regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,18840696], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • repressed by [protein|search|Rok] [Pubmed|15743949]
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    Biological materials

    Mutant

  • MGNA-B687 (yxaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1686 NBRP B. subtilis, Japan]
  • BKE39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA
  • BKK39940 ([gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA

    • Expression vectors

  • pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stlke] lab
  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP824, available in [SW|Stlke] lab, [pubmed|20389117]

    • References

  • 12073041,10746760,15743949,18957862