yxaL [2017-06-06 09:01:49]

similar to Ser/Thr kinase, increases the processivity of the PcrA helicase

locus: BSU39940

pI: 6.38

mw: 43.72 kDa

protein length: 410 aa

gene length: 1230 bp

function: unknown

product: unknown

essential: no

synonyms: yxaK

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 4,102,429 → 4,103,661

The protein

[SW|Localization]

• extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|0649520164CCC6120C40CE15EF08994BE0E79A25|yxaJ]-[gene|3BDC62A14BEEAEFD8E075E0958CAE41377808922|yxaL]
  description: [Pubmed|10746760]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,18840696], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by [protein|search|Rok] [Pubmed|15743949]

Biological materials

Mutant

• MGNA-B687 (yxaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1686 NBRP B. subtilis, Japan]

Expression vector

• pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
• for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP824, available in [SW|Stülke] lab

References

• 12073041,10746760,15743949,18957862