phoB

alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

Locus: BSU_05740

Isoelectric point: 5.89

Molecular weight: 50.36 kDa

Protein length: 462 aa

Gene length: 1389 bp

Function: aquisition of phosphate upon phosphate starvation, ether lipid synthesis

Product: alkaline phosphatase III, heptaprenylglyceryl phosphate processing phosphatase

Essential: no

E.C.: 3.1.3.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Secreted proteins

Gene

Coordinates: 620,097 621,485

The protein

Catalyzed reaction/ biological activity

• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• alkaline phosphatase family (with PhoA, according to UniProt)

Paralogous protein(s)

• PhoA

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Structure

• 3A52 (from Shewanella sp., 41% identity) PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: phoB-ydhF
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigA: sigma factor, in SigA regulon

  Regulatory mechanism

  • PhoP: activation, PubMed, in PhoP regulon

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

Biological materials

Mutant

• 1A767 ( phoB::cat), PubMed, available at BGSC
• BKE05740 (phoB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC
• BKK05740 (phoB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTGGATAACCTCCTAAA, downstream forward: _UP4_TAAAAGAATACAAGGTGTCC

References

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