ribonucleoside-diphosphate reductase (major subunit), control of DNA/cell mass ratio
function
synthesis of deoxyribonucleoside triphosphates
product
ribonucleoside-diphosphate reductase (major subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.5|Biosynthesis/ acquisition of nucleotides/ other][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|NrdR regulon], [SW|ResD regulon]Gene
Coordinates on the chromosome (coding sequence)
1,868,969 -> 1,871,071
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside diphosphate thioredoxin disulfide H2O = ribonucleoside diphosphate thioredoxin (according to Swiss-Prot)Protein family
ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|YosN]Effectors of protein activity
ATP stimulates CDP reduction. [Pubmed|24401092]dATP at concentrations up to ~10 µM stimulate CDP reduction, while higher concentrations inhibit this activity. [Pubmed|24401092]Structure
[PDB|1PEM] (from ''S. typhimurium'', 45% identity) [Pubmed|12818204][SW|Interactions]
[protein|search|NrdE]-[protein|search|NrdF] [Pubmed|16885274][protein|search|NrdE]-[protein|search|YosR] [Pubmed|24401092][protein|search|NrdE]-[protein|search|TrxA] [Pubmed|24401092]Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]Expression and Regulation
Operon
''[protein|search|nrdI]-[protein|search|nrdE]-[protein|search|nrdF]-[protein|search|ymaB]'' [Pubmed|16885274][SW|Sigma factor]
[protein|search|SigA] [Pubmed|16885274]Regulation
expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|16885274]Regulatory mechanism
[protein|search|ResD]: transcription activation [Pubmed|16885274][SW|NrdR]: transcription repression (predicted) [Pubmed|15949864]Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]Labs working on this gene/protein
[SW|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]References
Reviews
Original Publications
16885274,8969495,24401092,21561096,12818204,15949864 
