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nrdE [2017-06-19 17:29:32]
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nrdE [2017-06-19 17:29:32]

ribonucleoside-diphosphate reductase (major subunit), control of DNA/cell mass ratio
Locus
BSU17380
Isoelectric point
5.93
Molecular weight
80.50 kDa
Protein length
700 aa Sequence Blast
Gene length
2100 bp Sequence Blast
Function
synthesis of deoxyribonucleoside triphosphates
Product
ribonucleoside-diphosphate reductase (major subunit)
Essential
yes
E.C.
1.17.4.1
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
  • Groups of genes → Essential genes

    • Gene

    Coordinates
    1,868,969 → 1,871,071

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxyribonucleoside diphosphate thioredoxin disulfide H2O = ribonucleoside diphosphate thioredoxin (according to Swiss-Prot)
  • required for stationary-phase mutagenesis PubMed

    • Protein family

  • ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)

    • Paralogous protein(s)

  • NrdEB

    • Effectors of protein activity

  • ATP stimulates CDP reduction. PubMed
  • dATP at concentrations up to ~10 µM stimulate CDP reduction, while higher concentrations inhibit this activity. PubMed

    • Structure

  • 1PEM (from S. typhimurium, 45% identity) PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

    • Expression and Regulation

    Operons

    Genes
    nrdI-nrdE-nrdF-ymaB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • NrdR: repression, (predicted) PubMed, in NrdR regulon
  • PerR: transcription repression, PubMed, in PerR regulon

    • Regulation

  • expressed under anaerobic conditions (ResD) PubMed

    • Additional information

  • A ncRNA is predicted between 'ymzA' and 'nrdI' PubMed
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    Genes
    ymzA-nrdI-nrdE-nrdF-ymaB
    Description
    PubMed
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    Labs working on this gene/protein

  • Elisabeth Härtig, Braunschweig, Germany Homepage

    • References

    Reviews

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    Original Publications

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