SubtiBank SubtiBank
nrdE
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nrdE

ribonucleoside-diphosphate reductase (alpha subunit), control of DNA/cell mass ratio
Locus
BSU_17380
Isoelectric point
5.93
Molecular weight
80.50 kDa
Protein length
700 aa Sequence Blast
Gene length
2103 bp Sequence Blast
Function
synthesis of deoxyribonucleoside triphosphates
Product
ribonucleoside-diphosphate reductase (alpha subunit)
Essential
yes
E.C.
1.17.4.1
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
  • Groups of genes → Essential genes

    • Gene

    Coordinates
    1,868,969 1,871,071

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • [thioredoxin]-disulfide + 2'-deoxyribonucleoside 5'-diphosphate + H2O --> [thioredoxin]-dithiol + ribonucleoside 5'-diphosphate (according to UniProt)
  • required for stationary-phase mutagenesis PubMed

    • Protein family

  • ribonucleoside diphosphate reductase large chain family (with NrdEB, according to UniProt)

    • Paralogous protein(s)

  • NrdEB

    • Kinetic information

  • Steady-state kinetics PubMed

    • Effectors of protein activity

  • ATP stimulates CDP and UDP reduction PubMed
  • dATP at concentrations up to ~10 M stimulate CDP and UDP reduction, while higher concentrations inhibit this activity, inhibition by dATP is enhanced by binding of dAMP to the N-terminal domain of NrdE PubMed
  • TTP stimulates GDP reduction PubMed
  • dGTP stimulates ADP reduction PubMed

    • Structure

  • 6CGL (pH 4, dAMP bound) PubMed
  • 6CGM (pH 7, apo-protein) PubMed
  • 6CGN (pH 7, dAMP bound) PubMed
  • 6MT9 (TTP in substrate specificity site, ADP in inhibitory site (M-site), and ATP in activity site (I-site)) PubMed
  • 6MV9 (TTP in substrate specificity site, ADP in inhibitory site (M-site)) PubMed
  • 6MVE (TTP in S-site, ADP in M-site, ATP in I-site, and C-terminal cysteine residues captured in active site) PubMed
  • 6MW3 (cyro-EM structure of inhibitory filament formed with NrdF PubMed
  • 6MYX (cryo-EM structure of inhibitory filament) PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

    • Expression and Regulation

    Operons

    Genes
    nrdI-nrdE-nrdF-ymaB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • NrdR: repression, (predicted) PubMed, in NrdR regulon
  • PerR: transcription repression, PubMed, in PerR regulon

    • Regulation

  • expressed under anaerobic conditions (ResD) PubMed

    • Additional information

  • A ncRNA is predicted between 'ymzA' and 'nrdI' PubMed
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    Genes
    ymzA-nrdI-nrdE-nrdF-ymaB
    Description
    PubMed
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    Labs working on this gene/protein

  • Elisabeth Härtig, Braunschweig, Germany Homepage

    • References

    Reviews

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    Original Publications

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