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odhA [Fri Dec 09 2016 16:41:47 GMT+0100 (CET)]
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odhA [Fri Dec 09 2016 16:41:47 GMT+0100 (CET)]

2-oxoglutarate dehydrogenase (E1 subunit)
locus
BSU19370
pI
5.00
mw
105.00 kDa
function
TCA cycle
product
2-oxoglutarate dehydrogenase (E1 subunit)
essential
no
ec
1.2.4.2
synonyms
citK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,108,774 -> 2,111,608

    The protein

    Catalyzed reaction/ biological activity

  • 2-oxoglutarate [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine CO2 (according to Swiss-Prot)

    • Protein family

  • 2-oxoacid dehydrogenase family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Arg-66 and Arg-621 [Pubmed|22517742]

    • Structure

  • [PDB|2JGD] (from ''Escherichia coli'', 38% identity, 55% similarity) [Pubmed|17367808]

    • [SW|Interactions]

  • [protein|search|OdhA]-[protein|search|OdhB]-[protein|search|PdhD] [Pubmed|20933603]

    • Additional information

  • extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/2-Oxoglutarate_Dehydrogenase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|odhA]''-''[protein|search|odhB]'' [Pubmed|11976317]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1508153]

    • Regulation

  • repressed by glucose (2.4-fold) ([protein|search|CcpA]) [Pubmed|12850135]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|12850135]

    • Biological materials

    Mutant

  • GP671 (spc), GP684 (cat), GP1274 (cat) available in [SW|Jörg Stülke]'s lab
  • GP1274 (''[protein|search|odhA]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP1276 (''[protein|search|odhA]-[protein|search|odhB]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP2332 (''[protein|search|odhA]-[protein|search|odhB]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab
  • GP2334 (''[protein|search|odhA]-[protein|search|odhB]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 10672230

    • Original publications

  • 2500417,20933603,1508153,11976317,12850135,22517742,22900538,24263382