odhA

2-oxoglutarate dehydrogenase (E1 subunit)

Locus: BSU_19370

Isoelectric point: 5.87

Molecular weight: 105.54 kDa

Protein length: 944 aa

Gene length: 2835 bp

Function: TCA cycle

Product: 2-oxoglutarate dehydrogenase (E1 subunit)

Essential: no

E.C.: 1.2.4.2

Synonyms: citK

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 2,108,774 2,111,608

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ --> [dihydrolipoyllysine-residue succinyltransferase]-(R)-N⁶-(S⁸-succinyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

Protein family

• alpha-ketoglutarate dehydrogenase family (single member, according to UniProt)

Modification

• phosphorylated on Arg-66 and Arg-621 PubMed

Structure

• 2JGD (from Escherichia coli, 38% identity, 55% similarity) PubMed

Localization

• cytoplasm (according to UniProt)

Additional information

• extensive information on the structure and enzymatic properties of 2-oxoglutarate dehydrogenase can be found at Proteopedia

Expression and Regulation

Operons

• Genes: odhA-odhB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • repressed by glucose (2.4-fold) (CcpA) PubMed

Biological materials

Mutant

• GP671 (spc), GP684 (cat), available in Jörg Stülke's lab
• GP1274 Δ(odhA)::cat, available in Jörg Stülke's lab
• GP1276 Δ(odhA-odhB)::cat, available in Jörg Stülke's lab
• GP2183 Δ(odhA)::ermC, available in Jörg Stülke's lab PubMed
• GP2332 Δ(odhA-odhB)::kan, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
• GP2334 Δ(odhA-odhB)::lox72, Cre-recombinase is integrated in sacA, available in Jörg Stülke's lab
• BKE19370 (odhA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA
• BKK19370 (odhA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGAATATTACCCCCAA, downstream forward: _UP4_AAAAACTAAGGGGGAAATGA

LacZ fusion

• pGP3106 (in pAC7), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original publications

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