pbpA [2017-07-07 07:23:38]

class B penicillin-binding protein PBP 2A, required for cell wall synthesis during cell elongation

locus: BSU25000

pI: 9.57

mw: 79.96 kDa

protein length: 716 aa

gene length: 2148 bp

function: formation of a rod-shaped peptidoglycan cell wall, spore outgrowth

product: penicillin-binding protein PBP 2A

essential: no

synonyms: yqgF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,581,771 → 2,583,921

The protein

Catalyzed reaction/ biological activity

• required for [SW|cell wall synthesis] during cell elongation [Pubmed|12896990]
• driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] dynamics [Pubmed|21636744,21636745]

Structure

• [PDB|3VSK] (from Staphylococcus aureus, 45% identity)

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

Expression and Regulation

Operons

• genes: [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]
  description: [Pubmed|21815947]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

Labs working on this gene/protein

• [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

References

• 9851991,9139922,12896990,18957862,20487272,21636744,21636745,21815947,23895585