uvrA

excinuclease ABC (subunit A), required for transcription-dependent asymmetry in mutation rates of genes in the two orientations

Locus: BSU_35160

Isoelectric point: 5.84

Molecular weight: 105.83 kDa

Protein length: 957 aa

Gene length: 2874 bp

Function: DNA repair after UV damage

Product: excinuclease ABC (subunit A)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 3,610,064 3,612,937

Phenotypes of a mutant

• reduced stationary phase mutagenesis PubMed
• a uvrA uvrB mutant is sensitive to blue light-induced DNA damage PubMed
• sensitive to Cr(VI) treatment PubMed
• reduced resistance towards electron beams PubMed

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• 2 ABC transporter domains (aa 309-587, aa 607-935) (according to UniProt)

Structure

• 2R6F (Geobacillus stearothermophilus)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: uvrB-uvrA
  Description: PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

  Regulation

  • induced upon DNA damage (LexA) PubMed

  Additional information

  • during sporulation expressed both in the forespore and the mother cell PubMed
  • expression is induced in the presence of Cr(VI) PubMed

Biological materials

Mutant

• GP3534 (Δ(uvrA-uvrB)::kan), available in Jörg Stülke's lab
• GP1175 (Δ(uvrA-uvrB)::ermC), available in Jörg Stülke's lab PubMed
• BKE35160 (uvrA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG
• BKK35160 (uvrA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TATCCGATCCATAGCCATTT, downstream forward: _UP4_TAAAACCCTCTGTTAAGAGG

References

Reviews

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Original publications

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